1 edition of In vivo cellular imaging using fluorescent proteins found in the catalog.
Published
2012
by Humana Press in New York
.
Written in English
Edition Notes
Includes bibliographical references and index.
| Statement | edited by Robert M. Hoffman |
| Series | Methods in molecular biology -- 872 |
| The Physical Object | |
|---|---|
| Pagination | xiii, 269 p. |
| Number of Pages | 269 |
| ID Numbers | |
| Open Library | OL27094756M |
| ISBN 10 | 1617797960, 1617797979 |
| ISBN 10 | 9781617797965, 9781617797972 |
| OCLC/WorldCa | 801954028 |
Imaging cancer dynamics in vivo at the tumor and cellular level with fluorescent proteins Robert M. Hoffman Received: 19 December /Accepted: 3 August /Published online: 12 September . Probably triggered by the discovery and further engineering of increasing numbers of genetically encoded fluorescent proteins over the past years (Chalfie imaging at the cellular level usually requires .
Watching biological molecules provides clues to their function and regulation. Some of the most powerful methods of labeling proteins for imaging use genetically encoded fluorescent fusion tags. There are . The zebrafish visual system is a good research model because the zebrafish retina is very similar to that of humans in terms of the morphologies and functions. Studies of the retina have been facilitated by .
The fluorescent proteins we found to be optimal were not the same as in a comprehensive in vivo comparison of fluorescent proteins in yeast (Lee et al., ), suggesting some potential for organism . Imaging cellular dynamics with fluorescent proteins. Use of FPs has expanded into developmental models, such as zebrafish and Drosophila melanogaster, following the FP revolution of cell biology in .
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In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in.
In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in Format: Paperback. In vivo cellular imaging using fluorescent proteins - Methods and Protocols Article (PDF Available) in European journal of histochemistry: EJH 56(4):ejhbr14 October with 56 ReadsAuthor: Manuela Monti.
In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed. Get this from a library. In vivo cellular imaging using fluorescent proteins: methods and protocols.
[Robert M Hoffman;]. Get this from a library. In vivo cellular imaging using fluorescent proteins: methods and protocols. [Robert M Hoffman;] -- The discovery and genetic engineering of fluorescent proteins has revolutionized cell. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and.
Fluorescent proteins have enabled the color‐coding of cells growing in vivo. Noninvasive imaging of cells expressing fluorescent proteins has allowed the real‐time determination of the behavior of cancer Cited by: 9.
In vivo imaging has become quantifiable, highly sensitive and amenable to high-throughput study design. The high sensitivity and utility of in vivo imaging, is exemplified by use of the Fluorescence.
Abstract. Fluorescent proteins enable in vivo characterization of a wide and growing array of morphological and functional biomarkers. To fully capitalize on the spatial and temporal information. Fluorescent proteins as a toolkit for in vivo imaging Dmitriy M. Chudakov, Sergey Lukyanov and Konstantin A.
Lukyanov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho. Crestina L. Beites, William S.
Trimble, in Methods in Enzymology, Use of C-Terminal GFP-Tagged CDCrel-1 Fusion Protein to Monitor Septin Accumulation in vivo.
Green fluorescent protein. Keywords:Asthma, cancer, cellular dynamics, fluorescence imaging, GFP, infection, live mice, metastatic trafficking, nuclear dynamics, RFP, fluorescent-protein-expressing cells, visual drug targets.
Cellular labeling using NPs offers more possibilities for quantitative imaging of biological molecules (i.e., DNA, proteins, viruses, etc.) in the cell’s endogenous environment or detection of biospecific.
Fluorescent protein imaging is enabling not only whole-body imaging but, because of its cellular resolution and multiple colours, it is giving rise to a new field of in vivo cell biology. On the other hand, dynamic live cell imaging is already well developed especially in vivo using genetically introduced fluorescent proteins basically [25] [26][27][28][29][30][31] and some label.
Fluorescent proteins have revolutionized biology by enabling what was formerly invisible to be seen clearly, and potentially enable imaging of all cells in living animals.
There are numerous applications. In vivo imaging is a powerful tool used to study individual plasmids or protein-protein interactions in deep-tissue organs and whole mammals.
Genetically encoded fluorescent probes are suitable for stable. The present-day in vivo imaging community has been a key benefactor from the discovery of GFP, the characterization and cloning of the protein 8, along with the extension of the fluorescent.
Biliverdin-based fluorescent proteins [IFP, iRFP ] have opened new possibilities for imaging protein in deep tissue and in vivo using infrared excitation.
Other interesting developments. In vivo fluorescence imaging with near-infrared (NIR) light holds enormous potential for a wide variety of molecular diagnostic and therapeutic applications.
Because of its quantitative sensitivity, inherent .This detailed volume includes a rich variety of applications using various instrumentations, probes, disease models, and targets in order to account for the multidisciplinary nature of the use of in vivo fluorescence imagine.
The book .Also, in vivo systems can help researchers to better understand animal embryonic development so as how fluorescence proteins may be used to monitor different processes in living organisms at the Author: M.
Monti.
